Due to the large range of protein expression levels across different users and samples types we don’t recommend a specific dilution for every application. We recommend always diluting at least 1:2 using the diluent specified in the kit (usually Sample Diluent or Standard Diluent).
The Optimal Dilution is where the sample signal intensities fall in the middle of the dynamic range and should be determined by either:
1.) Prior to performing the full experiment, test a serially diluted representative sample from your cohort. Alternately, a small pool of several samples can also be used with this same method if sample volume is limited.
-or-
2.) Refer to published literature for expected concentrations and derive the optimal dilution level based on the expected dynamic range of the kit.
Need more help? Send us an email at techsupport@avivasysbio.com.
ELISA Kits by Research Area | View more ELISA Tips & Tricks
